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Qualitative proteomics analysis of the chicken amniotic fluid

ABSTRACT: We designed a bottom-up proteomic approach combining two complementary strategies as GeLC-MS/MS (protein samples fractionated by SDS-PAGE are analyzed by nanoLC-MS/MS after in-gel digestion) and shotgun (protein samples are directly analyzed by nanoLC-MS/MS after in-solution digestion) to give an exhaustive view of the proteome of the chicken amniotic fluid at the 11th day of the development, before the substantial transfer of egg white (which completely changes the global protein profile).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Amniotic Fluid

SUBMITTER: Lucie Combes-Soia

LAB HEAD: Rehault Godbert

PROVIDER: PXD008046 | Pride | 2018-02-21

REPOSITORIES: Pride

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The Unique Features of Proteins Depicting the Chicken Amniotic Fluid.

Da Silva Mylène M Dombre Clara C Brionne Aurélien A Monget Philippe P Chessé Magali M De Pauw Marion M Mills Maryse M Combes-Soia Lucie L Labas Valérie V Guyot Nicolas N Nys Yves Y Réhault-Godbert Sophie S

Molecular & cellular proteomics : MCP 20180214 Suppl 1

In many amniotes, the amniotic fluid is depicted as a dynamic milieu that participates in the protection of the embryo (cushioning, hydration, and immunity). However, in birds, the protein profile of the amniotic fluid remains unexplored, even though its proteomic signature is predicted to differ compared with that of humans. In fact, unlike humans, chicken amniotic fluid does not collect excretory products and its protein composition strikingly changes at mid-development because of the massive ...[more]

PMID: 29444982

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