Optimized NHS-amine labeling for multiplexed phosphoproteome study.

ABSTRACT: In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate that vacuum-drying in presence of hydroxylamine promotes β-elimination of phosphate groups from phosphoserine and phosphothreonine.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Brain Cancer

SUBMITTER: Yumi Kwon  

LAB HEAD: Cheolju Lee

PROVIDER: PXD008082 | Pride | 2018-02-27

REPOSITORIES: Pride