Proteomics

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Metaproteomics and direct Protein-SIF of the deep-sea mussel Bathymodiolus childressi and its bacterial symbionts

ABSTRACT: Bathymodiolus childressi is a species of deep-sea mussels found predominantly in the Gulf of Mexico. It colonizes cold seeps such as brine pool and oil seeps. The success of these animals in such environment is thought to be due to the symbiotic association of the mussel host with several species of bacteria. The aim of this study is to understand the role of the different partners involved in the symbiotic system using various “-omics” approaches. In addition to protein identification we used the mass spectrometry data generated and submitted with this project to derive the stable carbon isotope ratios for the different members of the symbiosis using the direct Protein-SIF method. The respective isotope pattern file and SIF computation files are included with this submission.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Archaea Bacteria Viruses Bathymodiolus Childressi

SUBMITTER: Tjorven Hinzke  

LAB HEAD: Manuel Kleiner

PROVIDER: PXD008089 | Pride | 2020-02-27

REPOSITORIES: Pride

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Publications

Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria).

Assié Adrien A   Leisch Nikolaus N   Meier Dimitri V DV   Gruber-Vodicka Harald H   Tegetmeyer Halina E HE   Meyerdierks Anke A   Kleiner Manuel M   Hinzke Tjorven T   Joye Samantha S   Saxton Matthew M   Dubilier Nicole N   Petersen Jillian M JM  

The ISME journal 20190927 1

Most autotrophs use the Calvin-Benson-Bassham (CBB) cycle for carbon fixation. In contrast, all currently described autotrophs from the Campylobacterota (previously Epsilonproteobacteria) use the reductive tricarboxylic acid cycle (rTCA) instead. We discovered campylobacterotal epibionts ("Candidatus Thiobarba") of deep-sea mussels that have acquired a complete CBB cycle and may have lost most key genes of the rTCA cycle. Intriguingly, the phylogenies of campylobacterotal CBB cycle genes suggest  ...[more]

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