Dataset Information

Proteomic analysis of Biomphalaria glabrata hemocyte during in vitro encapsulation of Schistosoma mansoi sporocycts

ABSTRACT: In order to provide a better understanding of molecular interactions/responses of snail host hemocyte and early-developing schistosome larvae (sporocyst stage) we perfomed comparative proteomic analyses on hemocytes during active encapsulation of sporocysts under in vitro conditions

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Schistosoma Mansoni (blood Fluke) Biomphalaria Glabrata

SUBMITTER: Greg Sabat

LAB HEAD: Dr. Timothy Yoshino

PROVIDER: PXD008135 | Pride | 2019-09-26

REPOSITORIES: Pride

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Publications

Proteomic Analysis of Biomphalaria glabrata Hemocytes During in vitro Encapsulation of Schistosoma mansoni Sporocysts.

Dinguirard Nathalie N Cavalcanti Marília G S MGS Wu Xiao-Jun XJ Bickham-Wright Utibe U Sabat Grzegorz G Yoshino Timothy P TP

Frontiers in immunology 20181129

Circulating hemocytes of the snail Biomphalaria glabrata, a major intermediate host for the blood fluke Schistosoma mansoni, represent the primary immune effector cells comprising the host's internal defense system. Within hours of miracidial entry into resistant B. glabrata strains, hemocytes infiltrate around developing sporocysts forming multi-layered cellular capsules that results in larval death, typically within 24-48 h post-infection. Using an in vitro model of ...[more]

PMID: 30555466

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Project description:Very little is known about how intervertebral disc (IVD) is formed or maintained. Members of the TGF-ß superfamily are secreted signaling proteins that regulate many aspects of development including cellular differentiation. We recently showed that deletion of Tgfbr2 in Col2a expressing tissue results in alterations in development of IVD annulus fibrosus. The results suggested TGF-ß has an important role in regulating development of the axial skeleton, however, the mechanistic basis of TGF-ß action in these specialized joints is not known. One of the hurdles to understanding development of IVD is a lack of known markers. To identify genes that are enriched in the developing IVD and to begin to understand the mechanism of TGF-ß action in IVD development, we undertook a global analysis of gene expression comparing gene expression profiles in developing vertebrae and IVD. We also compared expression profiles in tissues from wild type and Tgfbr2 mutant mice. Lists of IVD and vertebrae enriched genes were generated. Expression patterns for several genes were verified either through in situ hybridization or literature/ database searches resulting in a list of genes that can be used as markers of IVD. Cluster analysis using genes listed under the Gene Ontology terms multicellular organism development and pattern specification indicated that mutant IVD more closely resembled vertebrae than wild type IVD. We propose TGF-ß has two functions in IVD development: 1) to prevent chondrocyte differentiation in the presumptive IVD and 2) to promote differentiation of annulus fibrosus from sclerotome. We have identified genes that are enriched in the IVD and regulated by TGF-ß that warrant further investigation as regulators of IVD development. Thirteen samples were analyzed. This includes three biological replicates of laser captured IVD from E13.5 day control mice, three biological replicates of laser captured vertebrae from the same E13.5 day control mice, three biological relicates of laser captured vertebrae from E13.5 day Col2aCre;Tgfbr2lox/lox mice, and four biological replicates of laser captured IVD from E13.5 day Col2aCre;Tgfbr2lox/lox mice.

Dataset Information

Proteomic analysis of Biomphalaria glabrata hemocyte during in vitro encapsulation of Schistosoma mansoi sporocycts

Publications

Proteomic Analysis of <i>Biomphalaria glabrata</i> Hemocytes During <i>in vitro</i> Encapsulation of <i>Schistosoma mansoni</i> Sporocysts.

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