Proteomics

Dataset Information

0

The human cathelicidin antimicrobial peptide LL-37 promotes the growth of the pulmonary pathogen Aspergillus fumigatus.


ABSTRACT: Aspergillus fumigatus contributes to invasive and allergic pulmonary disease in immunocompromised individuals. The pulmonary mucus of cystic fibrosis (CF) patients displays elevated levels of the pleiotropic cathelicidin antimicrobial peptide LL-37 and the aim of this work was to assess the effect of LL-37 on the growth A. fumigatus. Exposure of A. fumigatus conidia to high concentrations of intact LL-37 (100 μg/ml) for 24 hours had a positive effect on growth (277.45 ± 22.59% (p < 0.01)) whereas scrambled LL-37 (76.45 ± 7.46%) did not. Exposure of 24 hour pre-formed mycelium to 5 μg/ml LL-37 for 48 hours increased hyphal wet weight significantly (4.37 ± 0.23 g, p < 0.001) compared to the control (2.67 ± 0.05 g). Amino acid leakage from mycelium was observed in the presence of LL-37 and gliotoxin secretion was increased at 24 hours from LL-37 exposed hyphae (169.1 ± 6.36 ng/mg hyphae, p < 0.05) compared to the control (102 ± 18.81 ng/mg hyphae). Quantitative proteomic analysis of 24 hour LL-37 treated hyphae revealed an increase in the abundance of proteins associated with growth (eIF-5A (16.3 fold increased), tissue degradation (aspartic endopeptidase (4.7 fold increased)) and allergic reactions (Asp F13 (10 fold increased)). By 48 hours there was an increase in proteins indicative of cellular stress (glutathione peroxidase (9 fold increased)), growth (eIF-5A (6 fold increased), and virulence (ribonuclease mitogillin (3.7 fold increased). These results indicate that LL-37 stimulates A. fumigatus growth and this may result in increased fungal growth and secretion of toxins in the lungs of CF patients.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Aspergillus Fumigatus Var. Fumigatus

SUBMITTER: Gerard Sheehan  

LAB HEAD: Kevin Kavanagh

PROVIDER: PXD008143 | Pride | 2018-05-22

REPOSITORIES: Pride

altmetric image

Publications

The Human Cathelicidin Antimicrobial Peptide LL-37 Promotes the Growth of the Pulmonary Pathogen Aspergillus fumigatus.

Sheehan Gerard G   Bergsson Gudmundur G   McElvaney Noel G NG   Reeves Emer P EP   Kavanagh Kevin K  

Infection and immunity 20180621 7


The pulmonary mucus of cystic fibrosis (CF) patients displays elevated levels of the cathelicidin antimicrobial peptide LL-37, and the aim of this work was to assess the effect of LL-37 on the growth of <i>Aspergillus fumigatus</i>, a common pathogen of CF patients. Exposure of <i>A. fumigatus</i> to LL-37 and its derived fragment RK-31 (1.95 μg/ml) for 24 h had a positive effect on growth (199.94% ± 6.172% [<i>P</i> < 0.05] and 218.20% ± 4.63% [<i>P</i> < 0.05], respectively), whereas scrambled  ...[more]

Similar Datasets

2017-06-17 | GSE98630 | GEO
2023-03-11 | PXD031565 | Pride
2023-03-10 | PXD030171 | Pride
2014-03-01 | GSE55467 | GEO
| E-GEOD-55467 | biostudies-arrayexpress
| E-GEOD-45177 | biostudies-arrayexpress
2016-03-11 | GSE45177 | GEO
2014-10-20 | GSE54810 | GEO
2020-03-19 | PXD016511 | Pride
2023-08-06 | GSE210767 | GEO