Proteomics

Dataset Information

0

Secretome of human chondrocytes and adipose mesenchymal stem cells


ABSTRACT: Objective: Assuming that mesenchymal stem cells adapt to the osteoarthritic joint environment to exert a chondroprotective effect, we aimed at investigating the molecular response set up by MSCs after priming by OA chondrocytes in cocultures. Design: We used primary human OA chondrocytes and adipose stem cells (ASCs) in mono- and cocultures and performed a high throughput secretome analysis. Among secreted proteins differentially induced in cocultures, we identified thrombospondin-1 (THBS1) as a potential candidate that could be involved in the chondroprotective effect of ASCs. Results: Secretome analysis revealed significant induction of THBS1in ASCs/chondrocytes cocultures at the mRNA and protein levels. Interestingly, we showed that THBS1 was up-regulated at late stages of MSC chondrogenic differentiation while recombinant THBS1 exerted a prochondrogenic effect on MSC. However, down-regulation of THBS1 in ASCs did not revert OA chondrocyte phenotype by decreasing hypertrophic and inflammatory markers. Nevertheless, down-regulation of THBS1 in ASCs reduced their immunosuppressive activity while recombinant THBS1 exerted an anti-inflammatory role on T lymphocytes. THBS1 function was evaluated in vivo in the collagenase-induced OA (CIOA) model by comparing ASCs expressing siTHBS1 and control ASCs. The OA protective effect of ASCs was reversed when THBS1 was down-regulated in ASCs indicating that THBS1 plays a role in the therapeutic effect of ASCs Conclusions: Our data gather some evidence that THBS1 exerts a pro-chondrogenic and anti-inflammatory function in vitro, which could partially explain a chondroprotective effect of ASCs in OA.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Articular Cartilage, Cell Culture, Mesenchymal Stem Cell Of Adipose, Articular Chondrocyte, Abdominal Adipose Tissue

DISEASE(S): Knee Osteoarthritis

SUBMITTER: marie maumus  

LAB HEAD: Danièle Noel

PROVIDER: PXD008146 | Pride | 2018-10-22

REPOSITORIES: Pride

altmetric image

Publications

Thrombospondin-1 Partly Mediates the Cartilage Protective Effect of Adipose-Derived Mesenchymal Stem Cells in Osteoarthritis.

Maumus Marie M   Manferdini Cristina C   Toupet Karine K   Chuchana Paul P   Casteilla Louis L   Gachet Mélanie M   Jorgensen Christian C   Lisignoli Gina G   Noël Danièle D  

Frontiers in immunology 20171129


<h4>Objective</h4>Assuming that mesenchymal stem cells (MSCs) respond to the osteoarthritic joint environment to exert a chondroprotective effect, we aimed at investigating the molecular response setup by MSCs after priming by osteoarthritic chondrocytes in cocultures.<h4>Methods</h4>We used primary human osteoarthritic chondrocytes and adipose stem cells (ASCs) in mono- and cocultures and performed a high-throughput secretome analysis. Among secreted proteins differentially induced in coculture  ...[more]

Similar Datasets

| E-GEOD-16464 | biostudies-arrayexpress
2009-09-02 | GSE16464 | GEO
| E-GEOD-64393 | biostudies-arrayexpress
| E-GEOD-64394 | biostudies-arrayexpress
| E-GEOD-61302 | biostudies-arrayexpress
2015-06-30 | GSE64394 | GEO
2015-06-30 | GSE64393 | GEO
2022-02-07 | GSE165874 | GEO
2021-05-10 | GSE171420 | GEO
| E-GEOD-10575 | biostudies-arrayexpress