Substrates of human TRAP complex

ABSTRACT: While the Sec61-complex in the membrane of the human endoplasmic reticulum facilitates translocation of all precursor polypeptides with amino-terminal signal peptides or transmembrane helices, the Sec61-associated translocon-associated protein (TRAP)-complex supports translocation of only a subset of precursor polypeptides, i.e. in a substrate-specific manner. To characterize TRAP-dependent precursors, we combined siRNA-mediated TRAP depletion in HeLa cells, label-free quantitative proteomics, and differential expression analysis. Sec61 served as a positive control

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nagarjuna Nagaraj  

LAB HEAD: Prof. Dr. Richard Zimmermann

PROVIDER: PXD008178 | Pride | 2018-08-09

REPOSITORIES: Pride