Proteomics

Dataset Information

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Determination of GPI structure of human CD59 in various KO CHO cells


ABSTRACT: To determine GPI structure of human CD59 in PGAP4-KO, PGAP3-KO, PGAP5-KO, and PGAP2-KO CHO cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Tetsuya Hirata  

LAB HEAD: Taroh Kinoshita

PROVIDER: PXD008230 | Pride | 2018-01-30

REPOSITORIES: Pride

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Publications

Identification of a Golgi GPI-N-acetylgalactosamine transferase with tandem transmembrane regions in the catalytic domain.

Hirata Tetsuya T   Mishra Sushil K SK   Nakamura Shota S   Saito Kazunobu K   Motooka Daisuke D   Takada Yoko Y   Kanzawa Noriyuki N   Murakami Yoshiko Y   Maeda Yusuke Y   Fujita Morihisa M   Yamaguchi Yoshiki Y   Kinoshita Taroh T  

Nature communications 20180126 1


Many eukaryotic proteins are anchored to the cell surface via the glycolipid glycosylphosphatidylinositol (GPI). Mammalian GPIs have a conserved core but exhibit diverse N-acetylgalactosamine (GalNAc) modifications, which are added via a yet unresolved process. Here we identify the Golgi-resident GPI-GalNAc transferase PGAP4 and show by mass spectrometry that PGAP4 knockout cells lose GPI-GalNAc structures. Furthermore, we demonstrate that PGAP4, in contrast to known Golgi glycosyltransferases,  ...[more]

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