Dataset Information

Protein-protein interactions overview of the moonlighting protein GAPDH in Paracoccidioides lutzii

ABSTRACT: we show an overview of experimental GAPDH interactome in different phases of P. lutzii and GAPDH interactions with macrophage proteins. Several proteins bound to GAPDH from mycelium, transition and yeast are common to important pathways such as glycolysis and TCA.

INSTRUMENT(S): Synapt MS, nanoACQUITY UPLC

ORGANISM(S): Paracoccidioides Lutzii (strain Atcc Mya-826 / Pb01) (paracoccidioides Brasiliensis)

SUBMITTER: Kleber Freitas

LAB HEAD: Maristela Pereira

PROVIDER: PXD008253 | Pride | 2019-07-29

REPOSITORIES: Pride

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Publications

Interactome of Glyceraldehyde-3-Phosphate Dehydrogenase Points to the Existence of Metabolons in <i>Paracoccidioides lutzii</i>.

E Silva Kleber Santiago Freitas KSF Lima Raisa Melo RM Baeza Lilian Cristiane LC Lima Patrícia de Sousa PS Cordeiro Thuany de Moura TM Charneau Sébastien S da Silva Roosevelt Alves RA Soares Célia Maria de Almeida CMA Pereira Maristela M

Frontiers in microbiology 20190709

<i>Paracoccidioides</i> is a dimorphic fungus, the causative agent of paracoccidioidomycosis. The disease is endemic within Latin America and prevalent in Brazil. The treatment is based on azoles, sulfonamides and amphotericin B. The seeking for new treatment approaches is a real necessity for neglected infections. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an essential glycolytic enzyme, well known for its multitude of functions within cells, therefore categorized as a moonlight protei ...[more]

PMID: 31338083

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Project description:Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. The PCM is a systemic disease caused by the thermo dimorphic fungus Paracoccidioides spp, which can exist as mycelia and yeast forms. The mycelia form can be found in environment at temperatures between 18 °C and 25 °C and produces spores or conidia. The PCM infection route occurs by the inhalation of conidia. Once in the lungs, these particles are converted into yeasts pathogenic form. Until now, metabolic aspects and virulence factors related to Paracoccidioides lutzii infective propagule are little understood. In this concern, a global proteomic study of P. lutzii conidia was performed. For conidia production, the mycelia of the isolate Pb01 were cultured in potato agar medium during 90 days at 18 °C. Obtained conidia were collected and purified. The proteins were extracted and subjected to tryptic digestion followed by identification by NanoUPLC-MSE approach. We identified a total of 242 proteins in P. lutzii conidia which were subjected to in silico functional analysis Proteins putatively acting as adhesins, such as GAPDH, enolase, and the glycoprotein β-1,3-glucanosyltransferase (gel2) were identified during our analysis. We also identified proteins related to signal transduction pathways, such as Ras and RhoA GTPases, previously demonstrated to be required for morphologic changes in pathogenic fungi. Proteins related to evasion, defense and virulence were also identified. Proteins acting during temperature shifts or oxidative stress provided by the host environment such as HSP90, catalase, mitochondrial peroxiredoxin Prx1 were identified. It were also identified proteins related to energy production and protein biosynthesis, which can provide important aspects to survival of this resting cells. These results highlight that P. lutzii conidia contain proteins that can contribute to its maintenance in the environment and also molecules related to host-pathogen interactions.

Dataset Information

Protein-protein interactions overview of the moonlighting protein GAPDH in Paracoccidioides lutzii

Publications

Interactome of Glyceraldehyde-3-Phosphate Dehydrogenase Points to the Existence of Metabolons in <i>Paracoccidioides lutzii</i>.

Similar Datasets

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