Dataset Information

Label free phosphoproteomic analysis of early phosphorylation events during deetiolation in Arabidopsis thaliana

ABSTRACT: Light environment provides signals for plants to develop and accomplish their life cycle successfully. Those signals are perceived and transduced by photoreceptors. Phosphorylation is one of the biochemical mechanisms initiating light signalling cascade and is a challenging question in the photobiology field today. Here, we study early light-induced phosphoproteome in Arabidopsis thaliana through a Label free LC-MS/MS proteomic approach to identify proteins which significantly change their phosphorylation status in a light-responsive way. And investigate the participation of the photoreceptors during light-dependent phosphoroylation changes.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seedling

SUBMITTER: María Agustina Mazzella

LAB HEAD: María Agustina Mazzella

PROVIDER: PXD008274 | Pride | 2022-02-15

REPOSITORIES: Pride

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Publications

A novel strategy to uncover specific GO terms/phosphorylation pathways in phosphoproteomic data in Arabidopsis thaliana.

Arico Denise S DS Beati Paula P Wengier Diego L DL Mazzella Maria Agustina MA

BMC plant biology 20211214 1

<h4>Background</h4>Proteins are the workforce of the cell and their phosphorylation status tailors specific responses efficiently. One of the main challenges of phosphoproteomic approaches is to deconvolute biological processes that specifically respond to an experimental query from a list of phosphoproteins. Comparison of the frequency distribution of GO (Gene Ontology) terms in a given phosphoproteome set with that observed in the genome reference set (GenRS) is the most widely used tool to in ...[more]

PMID: 34906086

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