Ontology highlight
ABSTRACT:
INSTRUMENT(S): Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
DISEASE(S): Breast Cancer
SUBMITTER: Tingting Jiang
LAB HEAD: Nicolas L. Young
PROVIDER: PXD008296 | Pride | 2018-07-05
REPOSITORIES: Pride
Proteomics 20180601 11
Post-translational modifications (PTMs) of histones are important epigenetic regulatory mechanisms that are often dysregulated in cancer. We employ middle-down proteomics to investigate the PTMs and proteoforms of histone H4 during cell cycle progression. We use pH gradient weak cation exchange-hydrophilic interaction liquid chromatography (WCX-HILIC) for on-line liquid chromatography-mass spectrometry analysis to separate and analyze the proteoforms of histone H4. This procedure provides enhanc ...[more]