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Dynamic changes in proteome and phosphoproteome following glutamate excitotoxicity in cultured primary cortical neurons

ABSTRACT: Excitotoxicity caused by over-stimulation of the ionotropic glutamate receptors is a key neuronal cell death process underpinning brain damage in acute and chronic neurological disorders such as ischaemic stroke, traumatic brain injury, and neurodegenerative diseases. Exactly how neurons die in excitotoxicity still remains unclear and is an important area of research in the field of neuroscience. In this current project we wanted to explore the global changes in proteome and phosphoproteome following glutamate excitotoxicity in cultured primary cortical neurons.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell Line Cell, Primary Neuron, Cell Culture

SUBMITTER: Ashfaqul Hoque

LAB HEAD: Associate Professor Heung-Chin Cheng

PROVIDER: PXD008353 | Pride | 2019-03-07

REPOSITORIES: Pride

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Quantitative proteomic analyses of dynamic signalling events in cortical neurons undergoing excitotoxic cell death.

Hoque Ashfaqul A Williamson Nicholas A NA Ameen S Sadia SS Ciccotosto Giuseppe D GD Hossain M Iqbal MI Oakhill Jonathan S JS Ng Dominic C H DCH Ang Ching-Seng CS Cheng Heung-Chin HC

Cell death & disease 20190301 3

Excitotoxicity, caused by overstimulation or dysregulation of ionotropic glutamate receptors (iGluRs), is a pathological process directing neuronal death in many neurological disorders. The aberrantly stimulated iGluRs direct massive influx of calcium ions into the affected neurons, leading to changes in expression and phosphorylation of specific proteins to modulate their functions and direct their participation in the signalling pathways that induce excitotoxic neuronal death. To define these ...[more]

PMID: 30824683

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