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Tissue Microfibril Comparison - Structural and compositional diversity of fibrillin microfibrils in human tissues

ABSTRACT: Fibrillin microfibrils are large supramolecular ECM assemblies which play different functional roles in different tissues. We aim to compare the ultrastructure and biological composition of fibrillin microfibrils purified from human eye, skin and cultured dermal fibroblasts. This will allow us to understand whether these their structure and compositions have evolved to suit the functions they play in different tissues.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ciliary Body, Skin, Fibroblast

SUBMITTER: Alexander Eckersley

LAB HEAD: Michael Sherratt

PROVIDER: PXD008450 | Pride | 2018-02-16

REPOSITORIES: Pride

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Structural and compositional diversity of fibrillin microfibrils in human tissues.

Eckersley Alexander A Mellody Kieran T KT Pilkington Suzanne S Griffiths Christopher E M CEM Watson Rachel E B REB O'Cualain Ronan R Baldock Clair C Knight David D Sherratt Michael J MJ

The Journal of biological chemistry 20180216 14

Elastic fibers comprising fibrillin microfibrils and elastin are present in many tissues, including the skin, lungs, and arteries, where they confer elasticity and resilience. Although fibrillin microfibrils play distinct and tissue-specific functional roles, it is unclear whether their ultrastructure and composition differ between elastin-rich (skin) and elastin-poor (ciliary body and zonule) organs or after <i>in vitro</i> synthesis by cultured cells. Here, we used atomic force microscopy, whi ...[more]

PMID: 29453284

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