ITRAQ - based proteomic analyses of sea cucumber Apostichopus japonicus in response to the stresses of heat, hypoxia and heat plus hypoxia
Ontology highlight
ABSTRACT:
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Stichopus Japonicus (sea Cucumber)
TISSUE(S): Respiratory System
SUBMITTER: 
Da Huo
LAB HEAD: Da Huo
PROVIDER: PXD008472 | Pride | 2019-01-07
REPOSITORIES: Pride
Publications
Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber Apostichopus japonicus.
Huo Da D Sun Lina L Zhang Libin L Ru Xiaoshang X Liu Shilin S Yang Xinyuan X Yang Hongsheng H
Journal of proteomics 20181221
Multiple environmental stressors caused by global warming influence the regulation of proteins involved in various biological processes in aquatic organisms. As an important component of the marine ecosystem, sea cucumber is a suitable echinoderm species for researching stress responses. In this study, we have investigated the proteomic response of respiratory trees in the sea cucumber Apostichopus japonicus, challenged with environmental stresses by using the iTRAQ technique. Results showed tha ...[more]
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The experiment performed in the laboratory also used vials with regular molasses and at 25C. Table I. Expedition log book for mount McKinley ascent. Information obtained during the ascent and summit of Mount McKinley, June 1st to June 16th of 2007. The oxygen pressure (PO2) was calculated from the barometric pressure. GNB means go and back from the mentioned point. DAY In order to understand the chronic hypoxia (CH) effect upon the absence of dystrophin, Drosophila melanogaster wild type and the model for DMD (dmDys), in which all dystrophins expression was knocked out by iRNA, were exposed to high altitude hypoxia (hypobaric hypoxia) during a 16-day climbing period reaching the summit of Mount McKinley (6194 meters above sea level). Furthermore, dmDys and Drosophila wild type were exposed to normobaric hypoxia (hypoxic chamber) following the same oxygen levels observed during the climbing expedition and to normoxic conditions for comparison. 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