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An integrated workflow for cross-linking/mass spectrometry

ABSTRACT: We present a concise workflow to enhance the mass spectrometric detection of cross-linked peptides by introducing sequential digestion and the database search software Xi. We use sequential digestion to reduce the peptide size and increase identification rates. The methodology is independent of samples complexity, cross-linker choice, method acquisition and can be used with multiple digestion steps.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Equus Caballus (horse) Homo Sapiens (human) Bos Taurus (bovine) Gallus Gallus (chicken) Chaetomium Thermophilum Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Bone Marrow Cell

SUBMITTER: Marta Mendes

LAB HEAD: Juri Rappsilber

PROVIDER: PXD008550 | Pride | 2019-08-27

REPOSITORIES: Pride

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