Proteomic analysis of cucumber (Cucumis sativus) fruit peel in response to pre-storage cold acclimation

ABSTRACT: To distinguish PsCA-responsive proteins, total proteins extracted from the non-acclimated fruit, the fruit exposed to 3 d of cold acclimation, the control fruit stored at 5oC for 12 d and the fruit exposed to 3d of PsCA treatment followed by 9 d of cold storage were separated by 2-DE, respectively. More than 750 protein spots were detected using PDQuest Advanced 2-D Gel Analysis software (Version 8.0) following the 2-DE gel analysis. Of the 750 spots, 58 that displayed significant and reproducible changes in abundance were isolated, and 56 spots were successfully characterized using MS/MS coupled with database searching, which matched 51 proteins. 38 proteins have scores greater than threshold scores of 61(P < 0.05) and they are regarded as the significant differentially- accumulated proteins (SDAPs).

INSTRUMENT(S):

ORGANISM(S): Cucumis Sativus

TISSUE(S): Skin

SUBMITTER: Shijiang Zhu  

LAB HEAD: Shijiang Zhu

PROVIDER: PXD008551 | Pride | 2018-10-22

REPOSITORIES: Pride