Protein and peptide turnover measurements using pulsed SILAC-TMT approach

ABSTRACT: We evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved investigation of protein stability.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Cervix

SUBMITTER: Jana Zecha  

LAB HEAD: Bernhard Kuster

PROVIDER: PXD008579 | Pride | 2018-02-09

REPOSITORIES: Pride