Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics

ABSTRACT: We investigated the compatibility of tube-gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxydative. To achieve this goal, we compared six extraction buffers in combination with two polymerization conditions to further optimize tube-gel protocol and evaluate its versatility.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Leslie MULLER  

LAB HEAD: Sarah CIANFERANI

PROVIDER: PXD008656 | Pride | 2018-06-06

REPOSITORIES: Pride