Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
DISEASE(S): Breast Cancer
SUBMITTER: Wenjing Peng
LAB HEAD: Yehia Mechref
PROVIDER: PXD008687 | Pride | 2018-05-16
REPOSITORIES: Pride
Zhu Rui R Zhou Shiyue S Peng Wenjing W Huang Yifan Y Mirzaei Parvin P Donohoo Kaitlyn K Mechref Yehia Y
Journal of proteome research 20180720 8
Protein glycosylation is a common protein post-translational modification (PTM) in living organisms and has been shown to associate with multiple diseases, and thus may potentially be a biomarker of such diseases. Efficient protein/glycoprotein extraction is a crucial step in the preparation of N-glycans derived from glycoproteins prior to LC-MS analysis. Convenient, efficient and unbiased sample preparation protocols are needed. Herein, we evaluated the use of sodium deoxycholate (SDC) acidic l ...[more]