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E. coli mirror LC-MS/MS - Precision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomics

ABSTRACT: Precision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomicsPrecision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomics

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli

SUBMITTER: Hao Yang

LAB HEAD: Ping Xu

PROVIDER: PXD008688 | Pride | 2019-01-11

REPOSITORIES: Pride

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Precision De Novo Peptide Sequencing Using Mirror Proteases of Ac-LysargiNase and Trypsin for Large-scale Proteomics.

Yang Hao H Li Yan-Chang YC Zhao Ming-Zhi MZ Wu Fei-Lin FL Wang Xi X Xiao Wei-Di WD Wang Yi-Hao YH Zhang Jun-Ling JL Wang Fu-Qiang FQ Xu Feng F Zeng Wen-Feng WF Overall Christopher M CM He Si-Min SM Chi Hao H Xu Ping P

Molecular & cellular proteomics : MCP 20190108 4

De novo peptide sequencing for large-scale proteomics remains challenging because of the lack of full coverage of ion series in tandem mass spectra. We developed a mirror protease of trypsin, acetylated LysargiNase (Ac-LysargiNase), with superior activity and stability. The mirror spectrum pairs derived from the Ac-LysargiNase and trypsin treated samples can generate full b and y ion series, which provide mutual complementarity of each other, and allow us to develop a novel ...[more]

PMID: 30622160

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