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HLA-DM-mediated HLA-DR3 Immunopeptidome

ABSTRACT: T2 cells stably expressing HLA-DR3 and variable amounts of HLA-DM (none, low, and high, per GFP co-expression) were lysed and HLA-DR3-peptide complexes were isolated via immunoprecipitation. Antigenic peptides were then eluted and quantified by LC-MS/MS to assess presented antigenic epitopes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Eliot Morrison

LAB HEAD: Christian Freund

PROVIDER: PXD008775 | Pride | 2018-04-16

REPOSITORIES: Pride

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Publications

Quantification of HLA-DM-Dependent Major Histocompatibility Complex of Class II Immunopeptidomes by the Peptide Landscape Antigenic Epitope Alignment Utility.

Álvaro-Benito Miguel M Morrison Eliot E Abualrous Esam T ET Kuropka Benno B Freund Christian C

Frontiers in immunology 20180503

The major histocompatibility complex of class II (MHCII) immunopeptidome represents the repertoire of antigenic peptides with the potential to activate CD4<sup>+</sup> T cells. An understanding of how the relative abundance of specific antigenic epitopes affects the outcome of T cell responses is an important aspect of adaptive immunity and offers a venue to more rationally tailor T cell activation in the context of disease. Recent advances in mass spectrometric instrumentation, computational po ...[more]

PMID: 29774024

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