Dataset Information

Proteomic study of Anton Chekhov' letters

ABSTRACT: Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in A. Chekhov’s museum in Melikhovo (nearby Moscow), have been analyzed by applying to these surfaces EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C8 resins) diskettes. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectrometry. The environmental microbiota present on samples and the M. ycobacterium Tuberculosis tuberculosis strain were described by a meta-proteomics approach. Eight identified M. Tuberculosis tuberculosis proteins confirmed the presence of the bacterium and the cause of Chekhov death, in addition to several sequenced peptides belonging to other bacterial species. The human plasma proteins and human keratins, detected on a tiny blood spot on the shirt, demonstrated the power of the combined approach.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mycobacterium Tuberculosis K

SUBMITTER: Alfonsina D'Amato

LAB HEAD: Alfonsina D'Amato

PROVIDER: PXD008786 | Pride | 2018-07-05

REPOSITORIES: Pride

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Publications

Anton Chekhov and Robert Koch Cheek to Cheek: A Proteomic Study.

D'Amato Alfonsina A Zilberstein Gleb G Zilberstein Svetlana S Golovan Mikhail Ivanovich MI Zhuravleva Anastasiya Anatolyevna AA Righetti Pier Giorgio PG

Proteomics 20180501 9

Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in the State Literary-Memorial Museum-Reserve A. P. Chekhov Melikhovo (nearby Moscow), have been analyzed by applying EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C<sub>8</sub> resins) diskettes to these surfaces. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectro ...[more]

PMID: 29522652

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Project description:The PhoPR two-component system is essential for virulence in Mycobacterium tuberculosis where it controls expression of approximately 2% of the genes, including those for the ESX-1 secretion apparatus, a major virulence determinant. Mutations in phoP lead to compromised production of pathogen-specific cell wall components and attenuation both ex vivo and in vivo. Using antibodies against the native protein in ChIP-seq experiments (chromatin immunoprecipitation followed by high-throughput sequencing) we demonstrated that PhoP binds to at least 35 loci on the M. tuberculosis genome. The PhoP regulon comprises several transcriptional regulators as well as genes for polyketide synthases and PE/PPE proteins. Integration of ChIP-seq results with high-resolution transcriptomic analysis (RNA-seq) revealed that PhoP controls 30 genes directly, whilst regulatory cascades are responsible for signal amplification and downstream effects. The most prominent site of PhoP regulation was located in the intergenic region between rv2395 and PE_PGRS41, where the mcr7 gene codes for a small non-coding RNA (ncRNA). Northern blot experiments confirmed the absence of Mcr7 in the M. tuberculosis phoP mutant as well as low-level expression of the ncRNA in M. tuberculosis complex members other than M. tuberculosis. By means of genetic and proteomic analyses we demonstrated that Mcr7 modulates translation of the tatC mRNA thereby impacting the activity of the Twin Arginine Translocation (Tat) protein secretion apparatus. As a result, secretion of the immunodominant Ag85 complex and the beta-lactamase BlaC is affected, among others. Mcr7, the first ncRNA of M. tuberculosis whose function has been established, therefore represents a missing link between the PhoPR two-component system and the downstream functions necessary for successful infection of the host. RNA-Seq: M. tuberculosis H37Rv (GC1237) cultures grown in vitro to exponential phase. One wild type sample plus one isogenic phoP mutant sample

Dataset Information

Proteomic study of Anton Chekhov' letters

Publications

Anton Chekhov and Robert Koch Cheek to Cheek: A Proteomic Study.

Similar Datasets

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