A Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE)

ABSTRACT: In the present study, we developed a novel targeted proteomics approach for quantification of allele-specific protein expression (ASPE) based on scheduled high resolution multiple reaction monitoring (sMRM-HR) with a heavy stable isotope-labeled quantitative concatamer (QconCAT) internal protein standard. This strategy was applied to the determination of the ASPE of UGT2B15 in human livers using the common UGT2B15 nonsynonymous variant rs1902023 (i.e. Y85D) as the marker to differentiate expressions from the two alleles. This novel ASPE approach has the potential to be widely utilized for the identification of cis-genetic variants capable of regulating gene expression at the protein level.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Jian Shi  

LAB HEAD: Hao-Jie Zhu, Ph.D.

PROVIDER: PXD008788 | Pride | 2018-08-28

REPOSITORIES: Pride