Peptidomics of Human Hypothalamic neurons

ABSTRACT: The study of peptide secretion, and identifying its control requires the use of ELISAs for measuring their levels pre and post stimulation. In place of ELISA's an LC-MS methdology was developed first to identify the correct peptides generated by the neurons, which could then be used to measure the peptide products by targetted LC-MS analysis. The Extraction was also performed on human hypothalamic tissue obtained from a tissue bank. Samples were analysed by nano LC-MS, and searched using Peaks 8.5.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Paraventricular Nucleus Of Hypothalamus, Cerebral Cortex, Hypothalamic Nucleus, Primary Cerebral Cortical Neuron�, Arcuate Nucleus

SUBMITTER: Richard Kay  

LAB HEAD: Florian Merkle

PROVIDER: PXD008795 | Pride | 2018-08-22

REPOSITORIES: Pride