Dataset Information

Proteomic profile of membrane vesicles of E. faecium

ABSTRACT: Vesicles were derived from 4 different E. faecium strains at 2 different growth conditions (exponential growth phase in nutrient rich medium / stationary growth in nutrient limiting medium). After purification of the vesicles the proteomic profile was obtained.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Enterococcus Faecium Do

SUBMITTER: Theresa Wagner

LAB HEAD: Mona Johannessen

PROVIDER: PXD008801 | Pride | 2018-06-10

REPOSITORIES: Pride

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Publications

Enterococcus faecium produces membrane vesicles containing virulence factors and antimicrobial resistance related proteins.

Wagner T T Joshi B B Janice J J Askarian F F Škalko-Basnet N N Hagestad O C OC Mekhlif A A Wai S N SN Hegstad K K Johannessen M M

Journal of proteomics 20180530

Enterococcus faecium is a commensal but also a bacteremia causing pathogen, which is inherently resistant to several antimicrobials and has a great ability to acquire new traits. Bacterial membrane vesicles (MVs) are increasingly recognized as a mode of cell-free communication and a way to deliver virulence factors and/or antimicrobial resistance determinants. These features make MVs interesting research targets in research on critical hospital pathogens. This study describes for the first time ...[more]

PMID: 29857065

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Project description:Enterococcus faecium has become a major opportunistic pathogen with the emergence of multidrug-resistant clones that are well-adapted to the hospital environment. As part of the vast diversity of gut microbiota, they are faced with different environmental stress, including antimicrobial pressure. By contrast, little is known about the effect of non-antibiotic molecules on bacterial physiology while numerous drugs are used in inpatients, especially those hospitalized in intensive care units (ICUs). The aim of this study was to investigate the impact of the most prescribed xenobiotics in ICUs on fitness, pathogenicity and antimicrobial resistance of E. faecium. Several phenotypic analysis was carried out and we rapidly brought to light that caspofungin, an antifungal agent belonging to the echinocandin family, seemed to have an important impact on E. faecium growth. Since the fungal target of caspofungin [beta-(1,3)-glucan synthase] is absent in enterococci, the mechanism of caspofungin action was investigated by several approaches. First, we decided to confirm this result by electronic microscopy and a peptidoglycan analysis by Ultra Performance Liquid Chromatography coupled with mass spectrometry (UPLC-MS/MS). Again, we highlighted that caspofungin even at subinhibitory concentrations (SICs) seemed to have an impact on cell wall organization especially in muropeptide precursors abundance. Then, a transcriptomic analysis was performed by RNA-seq (HiSeq 2500, Illumina) using the vanB-positive reference strain E. faecium Aus0004 in the presence or absence of caspofungin SIC (8 mg/L i.e., ¼ of the MIC). Transcriptomic analysis showed that the expression of 568 genes (19.9% of the genome) was significantly altered in the presence of caspofungin SIC, with 323 genes induced (fold change >2, p-value <0.1) and 245 genes repressed (fold change <-2, p-value <0.1). Regarding the repressed genes, the pdhABCD operon is largely downregulated (fold changes -4.3, -9.7, -6.9 and -6.4, respectively). This operon encoded components of the pyruvate deshydrogenase multienzyme complex involved in bacterial energetic pathway by the citrate cycle (i.e., TCA cycle). Moreover, it seemed that the glycerol metabolism pathway and in particular the glpOKF operon is downregulated too. The dramatic alteration of TCA seemed to have an drastic impact on bacterial cells viability indeed decrease of glycerol metabolism could explain the conformational modifications of peptidoglycan.

Dataset Information

Proteomic profile of membrane vesicles of E. faecium

Publications

Enterococcus faecium produces membrane vesicles containing virulence factors and antimicrobial resistance related proteins.

Similar Datasets

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