Proteomics

Dataset Information

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Pseudomonas aeruginosa PA3904 pull down coupled to LC-MS/MS

ABSTRACT: pETDUET His-PAAR4 and pETDUET Flag-PAAR4 were transformed into BL21 E. coli DE3 and expressed as described above.  An equal volume of PAO1 retS was grown to an OD600 of 2.0.  E. coli expressing His-PAAR4 or Flag-PAAR4 and Pseudomonas cells were harvested by centrifugation and lysed as described above.  Lysates of both species were combined and His-PAAR4 or Flag-PAAR4 were purified as before.  Eluted sample was mixed with SDS-loading buffer, boiled and run on a 12% SDS-PAGE for 0.6 cm.  After coomassie staining and de-staining, the resulting band was excised and used for LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Brianne Burkinshaw  

LAB HEAD: Tao Dong

PROVIDER: PXD008829 | Pride | 2018-03-09

REPOSITORIES: Pride

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Publications

A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex.

Burkinshaw Brianne J BJ   Liang Xiaoye X   Wong Megan M   Le Alexander N H ANH   Lam Linh L   Dong Tao G TG  

Nature microbiology 20180409 5

The type VI secretion system (T6SS) is used by many Gram-negative bacteria as a molecular weapon to modulate neighbouring bacterial and eukaryotic cells, thereby affecting the dynamics of community structure in multiple species environments. The T6SS injects its inner-needle Hcp tube, the sharpening tip complex consisting of VgrG and PAAR, and toxic effectors into neighbouring cells. Its functions are largely determined by the activities of its delivered effectors. Six mechanisms of effector del  ...[more]

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