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Differential regulation of tobacco host factors upon TMV infection

ABSTRACT: Tobacco mosaic virus (TMV) became one of the model virus for fundamental biological research and understanding of the host-pathogen interaction. It utilizes host machinery smartly to complete the life cycle, though many parts of life cycle remained a mystery, such as host factors involved in virus replication processes. We utilized the 8-Plex iTRAQ study to understand host-pathogen interaction between TMV and host tobacco.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Leaf

SUBMITTER: Prem Prakash Das

LAB HEAD: Prof Wong Sek Man

PROVIDER: PXD008837 | Pride | 2018-11-07

REPOSITORIES: Pride

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Comparative proteomics of Tobacco mosaic virus-infected Nicotiana tabacum plants identified major host proteins involved in photosystems and plant defence.

Das Prem Prakash PP Lin Qingsong Q Wong Sek-Man SM

Journal of proteomics 20181129

Tobacco mosaic virus (TMV) is a positive single-stranded RNA virus. Its 5' end ORF codes for the replicase proteins, namely 126 kDa and 183 kDa, respectively. These proteins interact with many host proteins to form a virus replication complex (VRC). This study aims to dissect the proteome profile of TMV-infected Nicotiana tabacum in host cellular and molecular pathways. We used the isobaric tags for relative and absolute quantification (iTRAQ) technique to analyse the differential global proteom ...[more]

PMID: 30503828

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