Dataset Information
High-resolution spatially-resolved proteome mapping using automated, sacrificial liquid-mediated sample transfer from laser microdissection to nanodroplet sample preparation
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: 
Matthew Monroe
LAB HEAD: Ryan T. Kelly
PROVIDER: PXD008844 | Pride | 2018-07-03
REPOSITORIES: Pride
Publications
Spatially Resolved Proteome Mapping of Laser Capture Microdissected Tissue with Automated Sample Transfer to Nanodroplets.
Molecular & cellular proteomics : MCP 20180624 9
Current mass spectrometry (MS)-based proteomics approaches are ineffective for mapping protein expression in tissue sections with high spatial resolution because of the limited overall sensitivity of conventional workflows. Here we report an integrated and automated method to advance spatially resolved proteomics by seamlessly coupling laser capture microdissection (LCM) with a recently developed nanoliter-scale sample preparation system termed nanoPOTS (Nanodroplet Processing in One pot for Tra ...[more]
