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Chromatin proteomics experiments for ProteomeHD

ABSTRACT: These is a collection of chromatin proteomics experiments that make up the 80 "in-house" biological conditions covered by ProteomeHD (see publication for more details). All experiments use SILAC, resulting in 80 SILAC ratios comparing the impact of various drugs (estradiol, TSA, hydroxytamoxifen, etc.), growth factors (TNFalpha) or other treatments (ionizing radiation) on chromatin composition. For a more detailed description see the Supplementary Table S2 of the publication, which can also be downloaded from here.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Diploid Cell

SUBMITTER: Georg Kustatscher

LAB HEAD: Juri Rappsilber

PROVIDER: PXD008888 | Pride | 2019-11-11

REPOSITORIES: Pride

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Co-regulation map of the human proteome enables identification of protein functions.

Kustatscher Georg G Grabowski Piotr P Schrader Tina A TA Passmore Josiah B JB Schrader Michael M Rappsilber Juri J

Nature biotechnology 20191104 11

Assigning functions to the vast array of proteins present in eukaryotic cells remains challenging. To identify relationships between proteins, and thereby enable functional annotation of proteins, we determined changes in abundance of 10,323 human proteins in response to 294 biological perturbations using isotope-labeling mass spectrometry. We applied the machine learning algorithm treeClust to reveal functional associations between co-regulated human proteins from ProteomeHD, a compilation of o ...[more]

PMID: 31690884

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