Protein quality control by MARCH6/TRC8
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
James Williamson
LAB HEAD: James Nathan
PROVIDER: PXD008955 | Pride | 2018-04-18
REPOSITORIES: Pride
Publications
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
Stefanovic-Barrett Sandra S Dickson Anna S AS Burr Stephen P SP Williamson James C JC Lobb Ian T IT van den Boomen Dick Jh DJ Lehner Paul J PJ Nathan James A JA
EMBO reports 20180308 5
Misfolded or damaged proteins are typically targeted for destruction by proteasome-mediated degradation, but the mammalian ubiquitin machinery involved is incompletely understood. Here, using forward genetic screens in human cells, we find that the proteasome-mediated degradation of the soluble misfolded reporter, mCherry-CL1, involves two ER-resident E3 ligases, MARCH6 and TRC8. mCherry-CL1 degradation is routed via the ER membrane and dependent on the hydrophobicity of the substrate, with comp ...[more]
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