Proteomics

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Proteomes of leaf growing zones in rice genotypes with contrasting drought tolerance


ABSTRACT: Plants require a distinctive cohort of enzymes to coordinate division and cell expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence we investigated proteins in tissues sampled from leaves of a drought-tolerant rice (IAC1131) to provide insights into the effect of soil drying on gene expression when compared with the drought-sensitive Nipponbare. Shoot growth zones were dissected to count dividing cells and extract protein for subsequent Tandem Mass Tags (TMT) quantitative proteomic analysis. Gene Ontology (GO) annotations of differentially expressed proteins provided insights into responses of Nipponbare and IAC1131 to drought. Soil drying did not affect the proportion of mitotic cells in IAC1131. More than 800 proteins across most functional categories were up-regulated in drought (and down-regulated on re-watering) in IAC1131, including those involved in organization of the meristem and subsequent cell formation. On the other hand, the proportion of dividing cells in Nipponbare was severely impaired during drought and fewer than 200 proteins responded in abundance when the growing zones underwent a drying cycle. However, those proteins involved in oxidation state and response to external stimuli were more likely to be upregulated by drought, even in Nipponbare.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza sativa  

TISSUE(S): Leaf

DISEASE(S): Not Available

SUBMITTER: Yunqi Wu  

LAB HEAD: Paul A. Haynes

PROVIDER: PXD008977 | Pride | 2019-03-26

REPOSITORIES: pride

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Publications

Proteomes of Leaf-Growing Zones in Rice Genotypes with Contrasting Drought Tolerance.

Wu Yunqi Y   Mirzaei Mehdi M   Pascovici Dana D   Haynes Paul A PA   Atwell Brian J BJ  

Proteomics 20190418 9


Plants require a distinctive cohort of enzymes to coordinate cell division and expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence, proteins in tissues sampled from leaves of a drought-tolerant rice (IAC1131) are investigated to provide insights into the effect of soil drying on gene expression relative to the drought-sensitive genotype Nipponbare. Shoot growth zones are dissected to estimate the proportion of dividing cells and extra  ...[more]

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