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XLinkProphet ReACT XL-MS - Increased sensitivity with automated validation of XL-MS cleavable peptide crosslinks

ABSTRACT: Control Data Crosslink ReACT Results Searched with Comet

INSTRUMENT(S): LTQ FT

ORGANISM(S): Equus Caballus (horse) Bos Taurus (bovine) Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Andrew Keller

LAB HEAD: James Bruce

PROVIDER: PXD009001 | Pride | 2019-07-12

REPOSITORIES: Pride

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Increased sensitivity with automated validation of XL-MS cleavable peptide crosslinks.

Keller Andrew A Chavez Juan D JD Bruce James E JE

Bioinformatics (Oxford, England) 20190301 5

<h4>Motivation</h4>Peptides crosslinked with cleavable chemical crosslinkers are identified with mass spectrometry by independent database search of spectra associated with the two linked peptides. A major challenge is to combine together the evidence of the two peptides into an overall assessment of the two-peptide crosslink.<h4>Results</h4>Here, we describe software that models crosslink specific information to automatically validate XL-MS cleavable peptide crosslinks. Using a dataset of cross ...[more]

PMID: 30137231

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