Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ, Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human) Pseudomonas Aeruginosa Pao1 Bacillus Subtilis
SUBMITTER: Ana Wang
LAB HEAD: Dennis Wolan
PROVIDER: PXD009004 | Pride | 2019-01-29
REPOSITORIES: Pride
Wang Ana Y AY Thuy-Boun Peter S PS Stupp Gregory S GS Su Andrew I AI Wolan Dennis W DW
Journal of proteome research 20180808 9
The lysis and extraction of soluble bacterial proteins from cells is a common practice for proteomics analyses, but insoluble bacterial biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble bacterial biomass of Gram<sup>-</sup> and Gram<sup>+</sup> bacteria can be rendered soluble. We use LC-MS/MS shotgun proteomics to show that bacterial proteins in the soluble and insoluble postlysis fractions differ significantly. Additionally, in the case of Gram<sup> ...[more]