Dataset Information

Proteomic characterization of Zamilon vitis virions produced in various contexts

ABSTRACT: Acanthamoeba-infecting Mimiviridae belong to three clades: Mimiviruses (A), Moumouviruses (B) and Megaviruses (C). The uniquely complex mobilome of these giant viruses includes virophages and linear 7 kb-DNA molecules called “transpovirons”. We recently isolated a new virophage (Zamilon vitis) and two new transpovirons (maBtv and mvCtv) respectively associated to B-clade and C-clade Mimiviridae. We used the capacity of the Zamilon virophage to replicate both on B-clade and C-clade host viruses to investigate the three partite interaction network governing the propagation of transpovirons. We notably performed proteomic comparisons of host viruses and virophage particles carrying or cleared of transpovirons in search of proteins involved in this adaptation process. These experiments revealed that transpoviron-encoded proteins are synthetized during the combined Mimiviridae/virophage/transpoviron replication process and are specifically incorporated into the virophage and Mimiviridae virions together with the cognate transpoviron DNA. This is a unique example of intricate commensalism in the viral world, where the Zamilon virophage and the transpoviron depend on their host giant virus to replicate, and the transpoviron depends on the virophage to propagate from one host virus to another.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Unclassified Virophages Mimiviridae

SUBMITTER: Yohann Couté

LAB HEAD: Virginie Brun

PROVIDER: PXD009037 | Pride | 2020-04-14

REPOSITORIES: Pride

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Publications

Exploration of the propagation of transpovirons within Mimiviridae reveals a unique example of commensalism in the viral world.

Jeudy Sandra S Bertaux Lionel L Alempic Jean-Marie JM Lartigue Audrey A Legendre Matthieu M Belmudes Lucid L Santini Sébastien S Philippe Nadège N Beucher Laure L Biondi Emanuele G EG Juul Sissel S Turner Daniel J DJ Couté Yohann Y Claverie Jean-Michel JM Abergel Chantal C

The ISME journal 20191210 3

Acanthamoeba-infecting Mimiviridae are giant viruses with dsDNA genome up to 1.5 Mb. They build viral factories in the host cytoplasm in which the nuclear-like virus-encoded functions take place. They are themselves the target of infections by 20-kb-dsDNA virophages, replicating in the giant virus factories and can also be found associated with 7-kb-DNA episomes, dubbed transpovirons. Here we isolated a virophage (Zamilon vitis) and two transpovirons respectively associated to B- and C-clade mim ...[more]

PMID: 31822788

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Dataset Information

Proteomic characterization of Zamilon vitis virions produced in various contexts

Publications

Exploration of the propagation of transpovirons within Mimiviridae reveals a unique example of commensalism in the viral world.

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