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A proteomic approach to study Brassica oleracea responses to black rot (Xanthomonas campestris pv. campestris), part 2

ABSTRACT: Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, ‘omic’ approaches are heralded as valuable technologies. In this work, combining iTRAQ technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc) and control plants at two different post-infection times were compared.

INSTRUMENT(S):

ORGANISM(S): Brassica Oleracea Var. Botrytis (cauliflower)

TISSUE(S): Leaf

SUBMITTER: valentina calamia

LAB HEAD: valentina calamia

PROVIDER: PXD009098 | Pride | 2022-02-23

REPOSITORIES: Pride

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			Action	DRS
	051114_Ecartea_Frac21_24_12.mzML	Mzml
	051114_Ecartea_Frac21_24_13.mzML	Mzml
	051114_Ecartea_Frac21_24_3.mzML	Mzml
	051114_Ecartea_Frac21_24_5.mzML	Mzml
	051114_Ecartea_Frac21_24_8.mzML	Mzml

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A proteomic approach to study Brassica oleracea responses to black rot (Xanthomonas campestris pv. campestris), part 1

Project description:Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, ‘omic’ approaches are heralded as valuable technologies. In this work, combining iTRAQ technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc) and control plants at two different post-infection times were compared.

2022-02-23 | PXD009097 | Pride

Transcriptomics of Xanthomonas campestris pv campestris in cabbage xylem sap

Project description:Xylem sap of young cabbage plantlets was recovered from root pressure exudation and used as a growth medium for the vascular pathogen Xanthomonas campestris pv campestris, the causative agent of the black rot of Brassicaceae.

2014-01-04 | E-MTAB-2290 | biostudies-arrayexpress

Early transcriptional response to phytopathogen Xanthomonas campestris pv. campestris by susceptible cabbage host

Project description:Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most devastating diseases of cruciferous crops worldwide. The pathogen infects and multiplies in plant vascular tissues and, as the disease progresses, the veins of infected tissues turn black and characteristic V-shaped lesions appear along the margins of leaves.The aim of this work is to identify differentially expressed genes from Brassica oleracea during early infection by Xcc, in an attempt to identify proteins related to resistance. Cabbge seedlings were inoculated with Xanthomonas campestris pv campestris (Xcc) suspension and cabbage gene expression at 6h., 24h. And 48h. After inoculation was assessed with help of Brassica 95k EST microarray chip.

2015-05-09 | E-GEOD-68670 | biostudies-arrayexpress

Transcriptomic response of Xanthomonas campestris pv. campestris after exposure to allyl-isothiocyanate and indol-3 carbinol

Project description:We performed a transcriptomic analysis of the necrotrophic bacteria Xanthomonas campestris pv. campestris exposed to two different isothiocyanates (allyl-isothiocyanate and indol-3-carbinol), searching for mechanisms of adaptation and detoxification of these chemicals.

2021-09-08 | GSE167555 | GEO

Early transcriptional response to phytopathogen Xanthomonas campestris pv. campestris by susceptible cabbage host

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Xanthomonas campestris pv. campestris 8004: wild type vs. ravA (or ravS or ravR) mutant

Project description:Transcriptional profiling of Xanthomonas campestris pv. campestris 8004 comparing control wild type strain with ravA (or ravS or ravR) mutant The effects of mutating ravS, ravR and ravA on EPS synthesis, biofilm production and motility were very different , the factors responsible for these differences are not clear. With comparative analysis of the regualtion pathways by RavS, RavR and RavA, we can indentify different genes regulated by these three genes and maybe explain the different phenotypes caused by these genes mutations.

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Small RNAs from Cuscuta campestris parasitizing Arabidopsis thaliana

Project description:Dodders (Cuscuta spp.) are obligate parasitic plants that obtain water and nutrients from the stems of host plants via specialized feeding structures called haustoria. Dodder haustoria facilitate bi-directional movement of viruses, proteins, and mRNAs between host and parasite, but the functional effects of these movements are not clear. Here we show that C. campestris haustoria accumulate high levels of many novel microRNAs (miRNAs) while parasitizing Arabidopsis thaliana hosts. Many of these miRNAs are 22 nts long, a usually rare size of plant miRNA associated with amplification of target silencing through secondary small interfering RNA (siRNA) production. Several A. thaliana mRNAs are targeted by C. campestris 22 nt miRNAs during parasitism, resulting in high levels of secondary siRNA production. The targeted mRNAs function in hormone perception, pathogen-defense signaling, phloem function, and stem-cell identity. Homologs of these target mRNAs from diverse plants also have high-confidence complementary sites to C. campestris miRNAs, suggesting that homologous mRNAs are targeted by C. campestris across its very broad host range. These data show that C. campestris miRNAs act as trans-species regulators of host gene expression, and suggest that they may act as virulence factors during parasitism.

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microRNA accumulation from Cuscuta campestris in vitro haustoria

Project description:The parasitic plant Cuscuta campestris produces specialized microRNAs that are specifically expressed at the haustorial interface. Some of these "Interface-Induced MicroRNAs" function to target host mRNAs. C. campestris haustoria can be induced in the absence of any host tissues using a combination of light and physical pressure. This experiment tested with such in vitro, host-free haustoria produced interface-induced microRNAs. Small RNA-seq was performed from three different treatments: Shoot tips of C. campestris without any haustoria formation, in vitro haustoria formed in the presence of host tissue (detached leaves of Arabidopsis thaliana) and in vitro haustoria formed in the absence of any host tissue.

2022-07-06 | GSE205256 | GEO

Full-length RNA-seq of short RNAs from Cuscuta campestris

Project description:Cuscuta campestris is an obligate parasitic plant that attaches to the stems of host plants to obtain water and nutrients. C. campestris produces a specialized set of microRNAs specifically at the host-parasite interface. Many of these interface-induced microRNAs target mRNAs from the host. This experiment was designed to capture full-length primary transcripts that give rise to C. campestris interface-induced microRNAs. C. campestris shoot tips were stimulated to produce haustoria, then harvested to extract total RNA. RNA was then treated with various enzymes to modify 5' ends. Libraries were then made and sequenced. Importantly the library method only captures RNAs with a 5'-monophosphate. Therefore the specific enzymatic treatments can reveal the native 5' ends of the sequenced RNAs. Data were used to identify the primary transcripts for many of the known C. campestris primary microRNA transcripts. The data were also interrogated to tally specific snRNAs (U1, U2, U4, and U5) that are known to have a 2,2,7mGppp cap, and the tally pre-tRNAs, which are known to have a ppp 5' end. The analyzed results indicated that C. campestris interface-induced microRNA primary transcripts likely have a 5' ppp, not a cap, consistent with transcription by RNA polymerase III. The analysis also demonstrated that C. campestris interface-induced microRNA primary transcripts mostly terminate within stretches of polyT and lack polyA tails, also features consistent with Pol III transcription. Finally, the analysis indicated that the transcriptional start sites were located ~ 55 bp downstream of a common ten-base pair promoter element (the upstream sequence element). The 55bp spacing is also consistent with Pol III transcription.

2023-01-03 | GSE221347 | GEO

Brassica nigra challenged by Pieris brassicae, eggs of Pieris brassicae, Xanthomonas campestris pv. raphani, Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae

Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated different single or dual stresses: the larvae Pieris brassicae, egg extract of Pieris brassicae, the bacterial Xanthomonas campestris pv. raphani, the aphid Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae.

2017-03-31 | E-MTAB-5059 | biostudies-arrayexpress

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