Proteomics

Dataset Information

0

Defining the cellular proteolytic landscape in systemic lupus erythematosus


ABSTRACT: Mass spectrometry analysis of cellular targets of protein degradation in PBMCs of healthy individuals and systemic lupus

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

DISEASE(S): Systemic Lupus Erythematosus

SUBMITTER: Assaf Kacen  

LAB HEAD: Yifat Merbl

PROVIDER: PXD009112 | Pride | 2018-10-11

REPOSITORIES: Pride

altmetric image

Publications


Cellular function is critically regulated through degradation of substrates by the proteasome. To enable direct analysis of naturally cleaved proteasomal peptides under physiological conditions, we developed mass spectrometry analysis of proteolytic peptides (MAPP), a method for proteasomal footprinting that allows for capture, isolation and analysis of proteasome-cleaved peptides. Application of MAPP to cancer cell lines as well as primary immune cells revealed dynamic modulation of the cellula  ...[more]

Similar Datasets

2018-10-11 | PXD009360 | Pride
2018-10-11 | PXD009438 | Pride
2020-01-29 | GSE144390 | GEO
2018-10-11 | PXD009181 | Pride
| E-GEOD-45923 | biostudies-arrayexpress
2014-12-08 | GSE45923 | GEO
| E-GEOD-38567 | biostudies-arrayexpress
| E-MEXP-1635 | biostudies-arrayexpress
2012-06-08 | GSE38567 | GEO
2018-03-21 | GSE112087 | GEO