Dataset Information

Proteomics analysis of human islets treated with IL-1b and IFN-g

ABSTRACT: Human islets were treated with IL-1b and IFN-g for 24h, digested with trypsin, multiplexed with TMT-10, fractionated by high pH reverse-phase chromatography and analyzed by tandem LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pancreatic Islet, Islet Of Langerhans

DISEASE(S): Type 1 Diabetes Mellitus

SUBMITTER: Ernesto Nakayasu

LAB HEAD: Tom Metz

PROVIDER: PXD009131 | Pride | 2019-10-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	BetaMarker_Cyto_24h_TMT10_F01_12Oct15_Arwen_15-07-13.raw	Raw
	BetaMarker_Cyto_24h_TMT10_F01_12Oct15_Arwen_15-07-13_pepXML.zip	Other
	BetaMarker_Cyto_24h_TMT10_F02_12Oct15_Arwen_15-07-13.raw	Raw
	BetaMarker_Cyto_24h_TMT10_F02_12Oct15_Arwen_15-07-13_pepXML.zip	Other
	BetaMarker_Cyto_24h_TMT10_F03_12Oct15_Arwen_15-07-13.raw	Raw

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Publications

Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.

Nakayasu Ernesto S ES Syed Farooq F Tersey Sarah A SA Gritsenko Marina A MA Mitchell Hugh D HD Chan Chi Yuet CY Dirice Ercument E Turatsinze Jean-Valery JV Cui Yi Y Kulkarni Rohit N RN Eizirik Decio L DL Qian Wei-Jun WJ Webb-Robertson Bobbie-Jo M BM Evans-Molina Carmella C Mirmira Raghavendra G RG Metz Thomas O TO

Cell metabolism 20200109 2

Type 1 diabetes (T1D) results from the progressive loss of β cells, a process propagated by pro-inflammatory cytokine signaling that disrupts the balance between pro- and anti-apoptotic proteins. To identify proteins involved in this process, we performed comprehensive proteomics of human pancreatic islets treated with interleukin-1β and interferon-γ, leading to the identification of 11,324 proteins, of which 387 were significantly regulated by treatment. We then tested the function of growth/di ...[more]

PMID: 31928885

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