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Modification of the ubiquitinome by Vps11 and Vps18

ABSTRACT: We quantified by SILAC the modification of the ubiquitinome in human HEK293T cells that overexpressed Vps11 or Vps18

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Kidney

DISEASE(S): Disease Free

SUBMITTER: Manfredo Quadroni

LAB HEAD: Didier Picard

PROVIDER: PXD009178 | Pride | 2019-04-18

REPOSITORIES: Pride

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Publications

Vps11 and Vps18 of Vps-C membrane traffic complexes are E3 ubiquitin ligases and fine-tune signalling.

Segala Gregory G Bennesch Marcela A MA Ghahhari Nastaran Mohammadi NM Pandey Deo Prakash DP Echeverria Pablo C PC Karch François F Maeda Robert K RK Picard Didier D

Nature communications 20190423 1

In response to extracellular signals, many signalling proteins associated with the plasma membrane are sorted into endosomes. This involves endosomal fusion, which depends on the complexes HOPS and CORVET. Whether and how their subunits themselves modulate signal transduction is unknown. We show that Vps11 and Vps18 (Vps11/18), two common subunits of the HOPS/CORVET complexes, are E3 ubiquitin ligases. Upon overexpression of Vps11/Vps18, we find perturbations of ubiquitination in signal transduc ...[more]

PMID: 31015428

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