Dataset Information
Defining the cellular proteolytic landscape cross time points, part 2
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture
SUBMITTER: 
Assaf Kacen
LAB HEAD: Yifat Merbl
PROVIDER: PXD009181 | Pride | 2018-10-11
REPOSITORIES: Pride
Publications
Revealing the cellular degradome by mass spectrometry analysis of proteasome-cleaved peptides.
Nature biotechnology 20181022
Cellular function is critically regulated through degradation of substrates by the proteasome. To enable direct analysis of naturally cleaved proteasomal peptides under physiological conditions, we developed mass spectrometry analysis of proteolytic peptides (MAPP), a method for proteasomal footprinting that allows for capture, isolation and analysis of proteasome-cleaved peptides. Application of MAPP to cancer cell lines as well as primary immune cells revealed dynamic modulation of the cellula ...[more]
