Dataset Information

Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes

ABSTRACT: Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This projected investigated molecular mechanisms of TEGDMA toxicity by identifying time- and dose dependent effects on the proteome of human THP-1 monocytes. Effects of different concentrations (0.07mM-5mM) and exposure times (0-72h) of TEGDMA on cell viability, proliferation, and morphology were determined by a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Cells were metabolically labelled (SILAC), and exposed to 0.3mM or 2.5mM TEGDMA for 6h or 16h prior to LC-MS/MS analyses. Regulated proteins were analysed with the STRING database. Cells exposed to 0.3mM TEGDMA showed increased viability, and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5mM TEGDMA showed diminished viability, and a protein expression profile associated with oxidative stress, DNA-damage, mitochondrial dysfunction and cell-cycle inhibition. Altered expression of immune genes was observed in both groups.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Jack-Ansgar Bruun

LAB HEAD: Ulf Örtengren

PROVIDER: PXD009206 | Pride | 2018-07-31

REPOSITORIES: Pride

ACCESS DATA

Publications

Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes.

Nilsen Bo W BW Simon-Santamaria Jaione J Örtengren Ulf U Jensen Einar E Bruun Jack-Ansgar JA Michelsen Vibeke B VB Sørensen Karen K KK

European journal of oral sciences 20180727 5

Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This study investigated the molecular mechanisms of TEGDMA toxicity by identifying its time- and dose-dependent effects on the proteome of human THP-1 monocytes. The effects of different concentrations (0.07-5 mM) and exposure times (0-72 h) of TEGDMA on cell viability, proliferation, and morphology were determined using a real-time viability assay, automated cell counting, and electron microscop ...[more]

PMID: 30051916

Similar Datasets

Project description:Carbon nanotubes (CNTs) are one of the most attractive nanomaterials that have many potential applications in industrial, medical, and environmental sectors. To investigate the toxic effect of single-walled CNTs (SWCNTs) on the immune system, we examined the influences of SWCNTs (depleted of metal impurities) on viability and global gene expression in murine macrophages. RAW264.7 Cells were exposed to various concentrations of SWCNTs, and cell viability was analyzed at various time points using WST-1 assay. SWCNT cytotoxicity in macrophages was concentration and time dependent, with significant viability loss occurring within 4 hr at 50Î¼g/ml SWCNT exposure. As low as 1Î¼g/ml SWCNTs reduced cell viability at 12 hr, whereas 50Î¼g/ml SWCNT exposure completely abolished cell proliferation. Laser confocal microscopy indicated that SWCNTs could enter and aggregate in cell cytoplasm and nuclear areas. Microarray, real-time reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay revealed that SWCNT altered gene and protein expression pattern. The most differentially expressed genes were related to proinflammatroy cytokines and chemokine, such as CCL-3 and CCL-4, apoptosis (e.g., Caspase-1, and -3), protective response such as Hmox-1and GSTM3) and cell surface molecules such as ICAM-1, Itgb2, indicating a possible involvement of SWCNT in inflammation and pulmonary granuloma formation, carcinogenesis and Th2 polarization of T cells. Furthermore, a substantial amount of down-regulated genes were related to ribosomal assembly and mitochondrial respiration chain, implicating a possible mechanism of SWCNT-induced oxidative stress and gene expression inhibition. The results are a first step in identifying molecular targets for the toxicity of SWCNTs and the elucidating of the molecular mechanisms for the immune modulation ability of SWCNTs. Cells were exposure to 50ug/ml of acid-functionzalized SWCNTs for 24 hours before gene expression profiling, cells without SWCNT exposure were used as control.

Dataset Information

Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes

Publications

Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure