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Proteome profiling in aging rat brain by LC-MS/MS

ABSTRACT: This study investigated proteome profile in the hippocampus, medial prefrontal cortex (mPFC), and striatum of 14, 18, 23, and 27 months old rats in order to see the effect of aging on proteins in these regions. Using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry, we identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins the striatum. Ninety-seven, 25, and 5 of these proteins were differentially expressed with age in the hippocampus, mPFC, and striatum, respectively. Overall, aging causes divergent protein changes in specific brain regions, with the most prominent changes observed in the late-aged.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Hamizah Shahirah Hamezah

LAB HEAD: Ikuo Tooyama

PROVIDER: PXD009212 | Pride | 2018-07-12

REPOSITORIES: Pride

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Proteome profiling in the hippocampus, medial prefrontal cortex, and striatum of aging rat.

Hamezah Hamizah Shahirah HS Durani Lina Wati LW Yanagisawa Daijiro D Ibrahim Nor Faeizah NF Aizat Wan Mohd WM Bellier Jean Pierre JP Makpol Suzana S Ngah Wan Zurinah Wan WZW Damanhuri Hanafi Ahmad HA Tooyama Ikuo I

Experimental gerontology 20180705

Decrease in multiple functions occurs in the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically in hippocampus, medial prefrontal cortex (mPFC), and striatum, can contribute to this age-associated decline in function. Our recent metabolomics analysis showed age-related changes in these brain regions. To further understand the aging processes, analysis using a proteomics approach was carried out. This study was conducted to ...[more]

PMID: 29981398

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