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C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2

ABSTRACT: Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear and chromosome biology.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Xin D. Gao

LAB HEAD: Erik J. Sontheimer

PROVIDER: PXD009216 | Pride | 2018-05-07

REPOSITORIES: Pride

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C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2.

Gao Xin D XD Tu Li-Chun LC Mir Aamir A Rodriguez Tomás T Ding Yuehe Y Leszyk John J Dekker Job J Shaffer Scott A SA Zhu Lihua Julie LJ Wolfe Scot A SA Sontheimer Erik J EJ

Nature methods 20180507 6

Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles. ...[more]

PMID: 29735996

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