Proteomics

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Absolute proteome quantification of highly purified reticulocytes and erythrocytes

ABSTRACT: Erythrocytes are the most numerous cells of the human body (Sender 2016). Their lifespan in the blood stream is around 120 days. The turnover implies the production of 2 million new reticulocytes each second. Circulating reticulocytes achieve their maturation in 2 to 3 days. During this short time, important modifications occur such as membrane remodeling, removing of remaining organelles and specific elimination of useless proteins such as the transferrin receptor. This remodeling will ensure the optimization of the erythrocyte ability to fulfil its transport functions. We performed a deep label-free quantitative proteome analysis of purified erythrocytes and reticulocytes to better understand the physiology of the erythrocytes and the last steps of their maturation.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Reticulocyte, Blood

SUBMITTER: Emilie-Fleur GAUTIER  

LAB HEAD: Patrick Mayeux

PROVIDER: PXD009258 | Pride | 2018-09-18

REPOSITORIES: Pride

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Absolute proteome quantification of highly purified populations of circulating reticulocytes and mature erythrocytes.

Gautier Emilie-Fleur EF   Leduc Marjorie M   Cochet Sylvie S   Bailly Karine K   Lacombe Catherine C   Mohandas Narla N   Guillonneau François F   El Nemer Wassim W   Mayeux Patrick P  

Blood advances 20181001 20

Reticulocytes produced in the bone marrow undergo maturation in the bloodstream to give rise to erythrocytes. Although the proteome of circulating red cells has been the subject of several reports, the cellular populations used for these studies were never completely devoid of reticulocytes. In our current study, we used highly purified erythrocyte and reticulocyte populations to quantify the absolute expression levels of the proteins in each cell population. Erythrocytes and reticulocytes were  ...[more]

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