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Proteomic analysis of E. coli K-12 LE392, P. putida KT2440 under exposure of carbamazepine

ABSTRACT: The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type E. coli K-12 LE392, P. putida KT2440, and their quantitative protein response under the exposure of antiepileptic drug carbamazepine. Three concentrations of carbamazepine were applied, which were 0.05 mg/L, 10.0 mg/L and 50.0 mg/L. The group without dosing carbamazepine was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of carbamazepine on bacterial translational levels can be revealed.

OTHER RELATED OMICS DATASETS IN: GSE112064

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Escherichia Coli Pseudomonas Putida Kt2440

TISSUE(S): Cell Culture

SUBMITTER: Jianhua Guo

LAB HEAD: Jianhua Guo

PROVIDER: PXD009279 | Pride | 2018-10-10

REPOSITORIES: Pride

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Antiepileptic drug carbamazepine promotes horizontal transfer of plasmid-borne multi-antibiotic resistance genes within and across bacterial genera.

Wang Yue Y Lu Ji J Mao Likai L Li Jie J Yuan Zhiguo Z Bond Philip L PL Guo Jianhua J

The ISME journal 20181005 2

Antibiotic resistance is a severe global threat for public health, causing around 700,000 deaths per year. Horizontal gene transfer (HGT) is one of the most significant pathways to disseminate antibiotic resistance. It is commonly acknowledged that sub-minimum inhibition concentrations of antibiotics are major contributors in promoting antibiotic resistance through HGT. Pharmaceuticals are occurring in our environments at increased levels, yet little is known whether non-antibiotic pharmaceutica ...[more]

PMID: 30291330

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