Proteomics

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Helpful dirt: Why diphtheria toxoid vaccines may also protect against non-toxigenic Corynebacterium diphtheriae strains


ABSTRACT: Diphtheria toxoid vaccines are among the oldest and safest vaccines known. The basic principle of production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins might be secreted by Corynebacterium diphtheriae, we assumed that diphtheria toxoid might not be the only component present in the vaccine. Therefore, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Western blot analyses indicated that these are immunogenic and may therefore support protection against C. diphtheriae. Furthermore, we could show that the vaccines also induce antibodies directed against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen evoking diphtheria-like illness and skin infections.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Clostridium Tetani E88 Corynebacterium Diphtheriae Nctc 13129

TISSUE(S): Cell Culture

SUBMITTER: Jens Möller  

LAB HEAD: Andreas Burkovski

PROVIDER: PXD009289 | Pride | 2019-04-17

REPOSITORIES: Pride

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Publications

More than a Toxin: Protein Inventory of <i>Clostridium tetani</i> Toxoid Vaccines.

Möller Jens J   Kraner Max Edmund ME   Burkovski Andreas A  

Proteomes 20190416 2


<i>Clostridium tetani</i> is the etiological agent of tetanus, a life-threatening bacterial infection. The most efficient protection strategy against tetanus is a vaccination with the <i>C. tetani</i> neurotoxin, which is inactivated by formaldehyde-crosslinking. Since we assumed that besides the tetanus toxin, other proteins of <i>C. tetani</i> may also be present in toxoid preparations, we analyzed commercially available vaccines from different countries in respect to their protein content usi  ...[more]

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