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I/L-discrimination by MS3 of dimethylated peptides

ABSTRACT: A novel mass spectrometric method for discriminating isoleucine and leucine by MS3. The dimethylation of peptide N-termini leads to intense a1 ions in CID and MS3 fragmentation of the isoleucine/leucine a1-ion leads to spectra with fragments that can discriminate between the two isomers. The method was applied to two antibodies in combination with a set of protease treatments using trypsin, thermolysin, chymotrypsin and pepsin to generate peptides exposing N-terminal I/L residues.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jakob Bunkenborg

LAB HEAD: Jakob Bunkenborg

PROVIDER: PXD009295 | Pride | 2018-07-24

REPOSITORIES: Pride

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