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Application of SPOT to Zn-treated HL-60 cells for multi-omics mechanism discovery, phosphoproteomics

ABSTRACT: Application of SPOT to Zn-treated HL-60 cells for multi-omics mechanism discovery. A time-resolved, multi-omics experiment using an automated, unified sample preparation platform. These datasets represent additional phosphoproteomics datasets(not prepared using SPOT) that were used along with the SPOT datasets for mechanism discovery

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Danielle Gutierrez

LAB HEAD: Richard M. Caprioli

PROVIDER: PXD009367 | Pride | 2018-11-21

REPOSITORIES: Pride

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An Integrated, High-Throughput Strategy for Multiomic Systems Level Analysis.

Gutierrez Danielle B DB Gant-Branum Randi L RL Romer Carrie E CE Farrow Melissa A MA Allen Jamie L JL Dahal Nikesh N Nei Yuan-Wei YW Codreanu Simona G SG Jordan Ashley T AT Palmer Lauren D LD Sherrod Stacy D SD McLean John A JA Skaar Eric P EP Norris Jeremy L JL Caprioli Richard M RM

Journal of proteome research 20180830 10

Proteomics, metabolomics, and transcriptomics generate comprehensive data sets, and current biocomputational capabilities allow their efficient integration for systems biology analysis. Published multiomics studies cover methodological advances as well as applications to biological questions. However, few studies have focused on the development of a high-throughput, unified sample preparation approach to complement high-throughput omic analytics. This report details the automation, benchmarking, ...[more]

PMID: 30114907

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