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Combining laser capture micro-dissection and proteomics reveals an active translation machinery controlling invadosome formation

ABSTRACT: We developed a method that combines laser micro-dissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this combinatorial method, we showed that invadosomes contain specific components of the translational machinery, in addition to known marker proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Dupuy Jean-William

LAB HEAD: Frédéric Saltel

PROVIDER: PXD009390 | Pride | 2018-10-19

REPOSITORIES: Pride

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Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation.

Ezzoukhry Zakaria Z Henriet Elodie E Cordelières Fabrice P FP Dupuy Jean-William JW Maître Marlène M Gay Nathan N Di-Tommaso Sylvaine S Mercier Luc L Goetz Jacky G JG Peter Marion M Bard Frédéric F Moreau Violaine V Raymond Anne-Aurélie AA Saltel Frédéric F

Nature communications 20180523 1

Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher their molecular composition, to understand their mechanisms, and to find specific elements to target them. However, the specific analysis of invadosomes is challenging, because it is difficult to maintain their integrity during isolation. In addition, classical purification methods often suffer from contaminations, which m ...[more]

PMID: 29795195

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