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Fibroblasts grown in 3D - Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts

ABSTRACT: Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigelTM amenable to stable isotope labeling by amino acids in cell culture and quantitative mass spectrometry-based proteomics analyses. We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAFs) on 3D culture conditions

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Skin, Fibroblast

DISEASE(S): Head And Neck Squamous Cell Carcinoma

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD009424 | Pride | 2018-09-10

REPOSITORIES: Pride

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Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts.

Tölle Regine C RC Gaggioli Cedric C Dengjel Jörn J

Journal of proteome research 20180723 8

In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology. However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results. Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and ar ...[more]

PMID: 29989826

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