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Spatial connectomics to identify agents relevant to lignocellulose deconstruction in fungi

ABSTRACT: Characterization of secretome of two white rot and two brown rot fungal species grown on aspen. Goal is to utilize temporal sampling to assist in elucidating lignocellulose degradation mechanism

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Postia Placenta Gloeophyllum Trabeum (brown Rot Fungus) Trametes Versicolor Serpula Lacrymans

TISSUE(S): Wood

SUBMITTER: Matthew Monroe

LAB HEAD: Jonathan Schilling

PROVIDER: PXD009480 | Pride | 2018-07-16

REPOSITORIES: Pride

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Publications

Coupling Secretomics with Enzyme Activities To Compare the Temporal Processes of Wood Metabolism among White and Brown Rot Fungi.

Presley Gerald N GN Panisko Ellen E Purvine Samuel O SO Schilling Jonathan S JS

Applied and environmental microbiology 20180801 16

Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressively colonize wood. Here, we applied secretomics in the same wafer design to track temporal trends on aspen decayed by fungi with distinct nutritional modes: two brown rot (BR) fungi ...[more]

PMID: 29884760

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